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. 2022 May 11;16:856734. doi: 10.3389/fncel.2022.856734

FIGURE 6.

FIGURE 6

Neurotrophin signaling is decreased in Sort1–/– Schwann cells. (A) pAkt, pMAPK, pRSK, and pCREB were quantified by densitometry of immunoblots from lysates of NT-3 or BDNF stimulated wild-type (WT) or Sort1–/– primary cultures of Schwann cells and normalized against β-actin. pMAPK, pRSK, and pCREB levels are significantly lower in Sort1–/– Schwann cells compared to WT Schwann cells. Data represent the mean ± SEM for n = 3. (B) The relative wound healing closure time was similar in Sort1–/– Schwann cells relative to WT Schwann cells with or without 10% FBS (positive control) or NT-3. Furthermore, the relative wound healing closure time indicates that NT-3 does not increase the relative wound healing closure time significantly in WT Schwann cells compared to unstimulated controls; 10% FBS significantly increased the relative wound healing closure time at 27–39 h (*p < 0.05). (C) Sort1–/– Schwann cells showed similar myelin formation relative to WT Schwann cells. Co-cultures of DRG neurons and Schwann cells were fixed 11 days after myelin induction with or without NT-3 stimulation. The myelin segments were identified with an antibody against myelin basic protein (MBP; green) and the neurites were identified with antibodies against neurofilament (red). Hoechst identifies nuclei (blue). Statistical significance was determined by two-way ANOVA with Bonferroni’s multiple comparisons post hoc test. Data represents mean ± SEM, n = 3 (*p < 0.05, **p < 0.005, ***p < 0.001, ****p < 0.0001).