Table I.
Real-time polymerase chain reaction primer sequences.
| RefSeq accession ID | Full gene name | Gene | Forward primer sequence 5' → 3' | Reverse primer sequence 5' → 3' |
|---|---|---|---|---|
| NM_001177520.3 | Alkaline phosphatase, biomineralization associated | ALPL | ACCACCACGAGAGTGAACCA | CGTTGTCTGAGTACCAGTCCC |
| NM_199173.6 | Bone gamma-carboxyglutamate protein / Osteocalcin | BGLAP | GAAGCCCAGCGGTGCA | CACTACCTCGCTGCCCTCC |
| NM_001200.4 | Bone morphogenic protein type 2 | BMP2 | AACACTGTGCGCAGCTTCC | CTCCGGGTTGTTTTCCCAC |
| NM_000088.4 | Collagen type 1 | COL1A1 | ACGTCCTGGTGAAGTTGGTC | ACCAGGGAAGCCTCTCTCTC |
| NM_000194.3 | Hypoxanthine phosphoribosyltransferase 1 | HPRT1 | CGAGATGTGATGAAGGAGATGG | GCAGGTCAGCAAAGAATTTATAGC |
| NM_002546.4 | Tumour necrosis factor (TNF) receptor super-family member 11b / Osteoprotegerin |
OPG | GCTCACAAGAACAGACTTTCCAG | CTGTTTTCACAGAGGTCAATATCTT |
| NM_003701.4 | Receptor activator of NF-κB ligand | RANKL | CATCCCATCTGGTTCCCATAA | GCCCAACCCCGATCATG |
| NM_001015051.3 | Runt-related transcription factor 2 | RUNX2 | ATACCGAGTGACTTTAGGGATGC | AGTGAGGGTGGAGGGAAGAAG |
| NM_003118.4 | Secreted protein acidic and rich in cysteine | SPARC | ATCTTCCCTGTACACTGGCAGTTC | CTCGGTGTGGGAGAGGTACC |