TABLE 1.

Oligonucleotide primers and fluorogenic probe for the detection of L. monocytogenes hlyA

Oligonucleotide	Sequence (5′→3′)a	Tm (°C)b	Positions (bp)c
HLYP1	CCTAAGACGCCAATCGAAAAGAAA	68	1633–1656
HLYP2	TAGTTCTACATCACCTGAGACAGA	68	2467–2490
HLYP8	AGGATTGGATTACAATAAAAACAA	60	1686–1709
HLYP9	TTCCGAATTCGCTTTTACGAGAGC	70	1873–1896
HLYP4R	CTTCTTCTTGCATTTTCCCTTC	62	2209–2230
HLYAP15d	RCGGAGATGCAGQGACAAATGTGCCp	72	1725–1741

^aR, FAM; Q, TAMRA; p, 3′ phosphate. 

^bT_m, melting temperature estimated from the formula 2(A+T) + 4(G+C) (45). Dyes are not accounted for in the calculations. 

^cPositions based on GenBank accession no. M24199 and M29030.