Figure 3.

Lung-recruited primary human monocytes induce proinflammatory signaling after transmigration across a SARS-CoV-2-infected epithelium

(A) Schematic diagram of the monocyte transmigration process: HLE-ALI cells were differentiated at ALI for two weeks and infected with SARS-CoV-2 at an MOI of 0.1 for 48 h, after which 10⁶ monocytes were transmigrated across the infected epithelium toward leukotriene B4 (LTB4) (100 nM) and C-C motif chemokine ligand 2 (CCL2) (250 pg/mL) for 24 h, in the absence or presence of remdesivir (1 μM) and/or baricitinib (1 μM). All conditions contained 0.1% v/v DMSO.

(B) Heatmaps of selected genes from each treatment condition. Monocyte mRNA was analyzed by multiplexed qRT-PCR and first normalized to GAPDH using the delta delta Ct method and then Z score normalized.