Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 25;39(11):110945. doi: 10.1016/j.celrep.2022.110945

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 The Author(s)

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

PMC Copyright notice

Lung-infiltrating monocytes release inflammatory mediators and harbor replicative SARS-CoV-2

(A) Inflammatory mediators in the apical fluid following transmigration were quantified by a multiplexed electrochemiluminescent assay. The Z score for each mediator in each condition was calculated and plotted.

(B) Transmigration efficiency of monocytes was calculated by dividing the number of monocytes in the apical fluid after 24 h by the input number of cells.

(C–E) RNA was extracted from each component of the model (epithelium, lung-infiltrating monocytes, and extracellular fluid) and reverse transcribed. Total SARS-CoV-2 genome copies were calculated.

(F) The sum of each of the components of the model was calculated to depict the total amount of virus remaining in the system after monocytes were allowed to transmigrate for 24 h.

All statistics were calculated using the Mann-Whitney U-test in Prism between the “no drug” and each treatment group. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001. Shown are median and interquartile range.