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. 2022 May 11;10:840964. doi: 10.3389/fcell.2022.840964

FIGURE 3.

FIGURE 3

Training zebrafish during an early period (3–14 dpl) increases adult neurogenesis in the rMP. (A). Experimental design. (B). Learning curves for Trained and Control individuals (Two-way RM ANOVA, Treatment effect: F(1, 14) = 126.1 with p < 0.0001, Session effect: F(9, 126) = 5.254 with p < 0.0001. Bonferroni’s multiple comparisons test, *** depicts p < 0.001, ****, p < 0.0001. Trained, N = 8; Control N = 8). (C). Simple linear regression for each training window for Trained fish (ANCOVA, F(1, 76) = 1.516, n. s. Trained, N = 8; Control N = 8). (D). BrdU+NeuN+ cell quantification in LP. (Two-way ANOVA, Treatment effect: F(1, 54) = 10.63 with p = 0.0019, Pallium region effect: F(3, 54) = 8.642 with p < 0.0001. Bonferroni’s multiple comparisons test, not significant differences. Trained, N = 8; Control N = 8). (E). Top: Neuronal fate (%BrdU+NeuN+/BrdU+ cells) in cLP. (Unpaired t test, t(14) = 4.749, *** depicts p < 0.001. Trained, N = 8; Control N = 8). Bottom: Cell migration in cLP (Mann Whitney test, U = 6492, n. s. Trained, N = 8; Control N = 8). (F). Adult-born neurons (BrdU+NeuN+) in cLP for Trained and Control individuals. Scale bar, 20 μm. (G). BrdU+NeuN+ cell quantification in MP. (Two-way ANOVA, Treatment effect: F(1, 56) = 15.34 with p = 0.0002, Pallium region effect: F(3, 56) = 2.685 with p = 0.0553. Bonferroni’s multiple comparisons test, * depicts p < 0.05. Trained, N = 8; Control N = 8). (H). Top: Neuronal fate (%BrdU+NeuN+/BrdU+ cells) in rMP (Unpaired t test, t(14) = 1.396, n. s. Trained, N = 8; Control N = 8). Bottom: Cell migration in rMP (Mann Whitney test, U = 2088, n. s. Trained, N = 8; Control N = 8). (I). Adult-born neurons (BrdU+NeuN+) in rMP for Trained and Control individuals. Scale bar, 20 μm. (I'). Higher magnification of the boxed square in I (merge panel). Single focal plane and orthogonal views after three-dimension reconstruction.