Table 1A.

Significantly upregulated proteins with Aquamin in high-fat mice.

		MS-NASH		C57BL6
Proteins	Genes	Aquamin	OCA	(Low-Fat)
UDP-N-acetylhexosamine pyrophosphorylase-like protein 1	Uap1l1	1.9 ± 0.8*	0.9 ± 0.4	1.1 ± 0.2
Biglycan	Bgn	1.7 ± 0.5*	1.5 ± 0.6	0.8 ± 0.3
Tax1-binding protein 3	Tax1bp3	1.6 ± 0.5*	1.6 ± 1.1	1.1 ± 0.5
Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial	Oxct1	1.6 ± 0.3*	1.4 ± 0.2*	1.2 ± 0.4
Glycogen phosphorylase, brain form	Pygb	1.5 ± 0.3*	0.9 ± 0.2	0.6 ± 0.1
Acyl-coenzyme A thioesterase 9, mitochondrial	Acot9	1.5 ± 0.4*	1.3 ± 0.6	0.8 ± 0.6
CD166 antigen	Alcam	1.5 ± 0.4*	1.3 ± 0.5	1.2 ± 0.2*
Erythrocyte band seven integral membrane protein	Stom	1.5 ± 0.4*	1.2 ± 0.5	0.9 ± 0.5
RNA-binding protein 3	Rbm3	1.5 ± 0.3*	1.4 ± 0.4*	1.2 ± 0.3
Angiotensinogen	Agt	1.5 ± 0.4*	1.1 ± 0.3	1.5 ± 0.4*
Apolipoprotein A-I	Apoa1	1.5 ± 0.3*	1.0 ± 0.3	0.8 ± 0.2
Heparin cofactor 2	Serpind1	1.5 ± 0.4*	1.2 ± 0.6	1.1 ± 0.4

These values (in this table) represent average (± standard deviation) fold-change of abundance ratios for each altered (upregulated) protein compared to the high-fat control group (MS-NASH mice on a high-fat diet) with a 1.5-fold change threshold in response to Aquamin intervention and are significant with a p-value <0.05 (*). For each upregulated protein with Aquamin, corresponding values from the other two groups are shown for comparison. These liver samples (from five mice in each group) were individually assessed by TMT-based differential proteomic expression and data were merged to get averages. Protein FDR Confidence for all proteins was ≤ 1%. Asterisks indicate statistical significance as compared to High-fat control at p <0.05. FDR, False Discovery Rate. These data are also presented in Figure 4.