Table 1C.
Significantly downregulated proteins with Aquamin in high-fat mice.
| MS-NASH | C57BL6 | |||
|---|---|---|---|---|
| Proteins | Genes | Aquamin | OCA | (Low-Fat) |
| Cytochrome P450 2C54 | Cyp2c54 | 0.48 ± 0.14* | 0.61 ± 0.19* | 2.72 ± 0.49 |
| Major intrinsically disordered NOTCH2-binding receptor 1-like homolog# | Minar2 | 0.49 ± 0.12* | 0.45 ± 0.18* | 1.06 ± 0.23 |
| Alpha-1-antitrypsin 1–5 | Serpina1e | 0.52 ± 0.27* | 0.70 ± 0.35 | 37.05 ± 11.87 |
| Protein CASC3 | Casc3 | 0.57 ± 0.13* | 0.55 ± 0.16* | 0.60 ± 0.22* |
| Major urinary protein 2 | Mup2 | 0.59 ± 0.23* | 0.44 ± 0.19* | 10.40 ± 5.30 |
| Cysteine dioxygenase type 1 | Cdo1 | 0.59 ± 0.18* | 0.54 ± 0.11* | 1.94 ± 0.66 |
| Sodium-coupled neutral amino acid transporter 4 | Slc38a4 | 0.60 ± 0.27* | 0.62 ± 0.27* | 1.30 ± 0.37 |
| Ferritin light chain 1 | Ftl1 | 0.62 ± 0.23* | 0.74 ± 0.27 | 1.18 ± 0.80 |
| 40S ribosomal protein S30 | Fau | 0.63 ± 0.33* | 0.71 ± 0.26* | 0.80 ± 0.42 |
| Sodium-coupled neutral amino acid transporter 3 | Slc38a3 | 0.63 ± 0.13* | 0.71 ± 0.18* | 2.60 ± 1.10 |
| 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase | Urad | 0.64 ± 0.20* | 0.94 ± 0.20 | 3.19 ± 2.07 |
| Cytochrome P450 2C50 | Cyp2c50 | 0.65 ± 0.22* | 0.79 ± 0.40 | 4.82 ± 2.53 |
| 5'-nucleotidase | Nt5e | 0.66 ± 0.28* | 0.67 ± 0.19* | 1.33 ± 0.30 |
| Ileal sodium/bile acid cotransporter | Slc10a2 | 0.67 ± 0.23* | 0.77 ± 0.22 | 0.54 ± 0.27* |
These values (in this table) represent average (± standard deviation) fold-change of abundance ratios for each altered (downregulated) protein compared to the high-fat control group (MS-NASH mice on a high-fat) with a 1.5-fold change threshold in response to Aquamin intervention and are significant with a p-value <0.05 (*). For each downregulated protein with Aquamin, corresponding values from the other two groups are shown for comparison. These liver samples (from five mice in each group) were individually assessed by TMT-based differential proteomic expression and data were merged to get averages. Protein FDR Confidence for all proteins was ≤ 1% except for 1 downregulated protein# ( ≤ 2%). Asterisks indicate statistical significance as compared to High-fat control at p < 0.05. FDR, False Discovery Rate. These data are also presented in Figure 4.