Figure 8.

Accelerated formation of vascular network and maturation of glomerular‐like structures in kidney organoids in vivo when transplanted with kidney dECM. a,b) Representative confocal images of MECA32 in transplanted graft. MECA32‐positive cells were more abundantly observed in kidney organoids transplanted with kidney dECM, indicating the effect of kidney dECM in recruiting endothelial cells from the mouse kidney into the transplanted graft. Scale bar = 200 µm (n = 6). Values are mean ± SEM. **p < 0.01, measured by t‐test. c) Representative confocal images of HNA (Human nuclei antibody), MECA32 and NPHS1 in kidney organoids in vivo when transplanted with kidney dECM. d) Representative confocal images of HNA, CD31, and Laminin in kidney organoids in vivo when transplanted with kidney dECM. e) Representative confocal images of slit diaphragm like structures in kidney organoids transplanted with kidney dECM. Scale bar = 50 µm. f) Representative confocal images of fluorescein isothiocyanate (FITC)‐labeled dextran present inside the vessels and capillaries of glomerular‐like structures in the transplanted kidney organoids. Scale bar = 50 µm. g) Representative transmission electron microscopy (TEM) images of kidney organoids in vitro, transplanted kidney organoids, kidney organoids transplanted with kidney dECM, and adult mouse kidney. Red arrowheads indicated podocyte and tubule maturation structures. Scale bar = 2 µm.