Figure 2.

EV are uptaken by endothelial cells and cardiomyocytes. A) Representative immunofluorescence images of the uptake assays performed in HUVEC and hiPSC‐CM. B) Effects of Dynasore treatment on EV uptake. Quantification of the average red channel intensity corresponding to the emission range of PKH26 in HUVEC treated with PKH26‐EV in the absence or presence of increasing concentrations of Dynasore. Data presented as mean ± SD, n = 3, *p < 0.05, **p < 0.01, ns: nonsignificant versus EV‐PKH26 group by one‐way ANOVA with Dunnett's multiple comparisons test, with a single pooled variance. C) Representative immunofluorescence images of the uptake inhibition assay performed in HUVEC and hiPSC‐CM upon addition of 50 × 10⁻⁶ m of Dynasore. HUVECs were stained for the transmembrane protein CD31 (green), EV were labeled with PKH26 and nuclei were counterstained with DAPI (blue). Cells were observed under an inverted fluorescence microscope (DMI6000, Leica Microsystems GmbH, Germany). Scale bar: 100 µm.