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. 2022 Mar 24;9(15):2104296. doi: 10.1002/advs.202104296

Figure 7.

Figure 7

Overexpression of a miRNA stemness cluster induces endothelial cell migration. A) Schematic representation of the transient transfection assays performed on human umbilical vein endothelial cells (HUVEC). B) Wound closure 24 h post‐scratch (48 h post‐transfection). Three of the five miRNA transfected into HUVEC were able to promote wound closure (measured as a percentage of the initial wound area; n = 4). C) Schematic overview of the described interactions of miRs‐200c‐3p, 363‐3p, and 302c‐3p with the PI3K/AKT pathway (Adobe Illustrator). D,E) Gene expression profiles of PTEN, CDKN1A (p21WAF/Cip1), and CCND1 (cyclin D1) at 24 and 48 h after HUVEC transient transfection. Data presented as mean ± SD, n = 4 for (B) and n = 3 for (D) and (E). Significance was tested against the scramble control. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s. (p > 0.05) by one‐way ANOVA with Dunnett's multiple comparisons test, with a single pooled variance.