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. 1999 May;65(5):2143–2150. doi: 10.1128/aem.65.5.2143-2150.1999

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Copyright © 1999, American Society for Microbiology

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FIG. 1 — (A) Agarose gel analysis of DNA extracted from aquifer field samples and standards. Lanes 1 to 3, Pseudomonas genomic DNA loaded at 0.59, 1.18, and 2.47 μg; lanes 4 and 5, aliquots (10 μl) of purified DNAs extracted from the NC or FC sediment (total volumes of the DNA extracts were 100 and 600 μl for the NC and FC extracts, respectively). (B) Relative abundance (universal probe-normalized signals) of domains and subgroups (phylum and subclass levels) in the NC and FC aquifer samples. The sums of domain and subgroup hybridization signals (± standard deviations) are given in the box above the graph. Legend for probe target groups: ■, universal; □, Bacteria; , Eucarya; , ALF; , B+G; , SRB; , HGC. Data are means of duplicate measurements (± standard deviations). An asterisk indicates that the hybridization signal from a given probe differed significantly (P ≤ 0.05) from that for the nonamended control.

FIG. 1 — (A) Agarose gel analysis of DNA extracted from aquifer field samples and standards. Lanes 1 to 3, Pseudomonas genomic DNA loaded at 0.59, 1.18, and 2.47 μg; lanes 4 and 5, aliquots (10 μl) of purified DNAs extracted from the NC or FC sediment (total volumes of the DNA extracts were 100 and 600 μl for the NC and FC extracts, respectively). (B) Relative abundance (universal probe-normalized signals) of domains and subgroups (phylum and subclass levels) in the NC and FC aquifer samples. The sums of domain and subgroup hybridization signals (± standard deviations) are given in the box above the graph. Legend for probe target groups: ■, universal; □, Bacteria; , Eucarya; , ALF; , B+G; , SRB; , HGC. Data are means of duplicate measurements (± standard deviations). An asterisk indicates that the hybridization signal from a given probe differed significantly (P ≤ 0.05) from that for the nonamended control.