TABLE 1.

Summary of sequences, target groups, and hybridization conditions for oligonucleotides used as phylogenetic probes

Probea	Sequence (5′ to 3′)	Target	Temp (°C)b	Reference
S-*-Univ-1390-a-A-18	GAC GGG CGG TGT GTA CAA	All organisms (universal)	54	44
S-D-Bact-0338-a-A-18	GCT GCC TCC CGT AGG AGT	Bacteria	42	4
S-D-Euca-1379-a-A-16	TAC AAA GGG CAG GGA C	Eucarya	40	22
S-D-Arch-0915-a-A-20	GTG CTC CCC CGC CAA TTC CT	Archaea	50	39
S-Sc-aProt-0019-a-A-17	CGT TCG (C/T)TC TGA GCC AG	α-Proteobacteria	47	31
L-Sc-bProt-1027-a-A-17	GCC TTC CCA CTT CGT TT	β-Proteobacteria	50	31
L-Sc-gProt-1027-a-A-17	GCC TTC CCA CAT CGT TT	γ-Proteobacteria	47	31
S-*-Srb-0385-a-A-18	CGG CGT CGC TGC GTC AGG	Sulfate-reducing bacteria	55	3
L-P-Grps-1901-a-A-18	TAT AGT TAC CAC CGC CGT	High-G+C gram positives	47	36
L-S-E. coli-1531-a-A-21	CAC CGT AGT GCC TCG TCA TCA	E. coli	50	34

^aNaming convention proposed by Alm et al. (1). 

^bEmpirically optimized for hybridization and membrane washes.