TABLE 2.
Microbial cultures used to prepare reference DNAs for initial optimization of probe hybridization conditions and routine probe calibration
| Taxonomic group and organism | Culture sourceb |
|---|---|
| Eucarya | |
| Penicillium crustosuma | 1 |
| Archaea | |
| Methanobacterium formicicuma | 2 |
| Bacteria | |
| Proteobacteria | |
| α | |
| Azospirillum brasilensea | 3 |
| Rhodospirillum rubrum | 4 |
| Sinorhizobium meliloti | 5 |
| Rhizobium loti | 5 |
| β | |
| Burkholderia cepacia | 6 |
| Alcaligenes sp.a | 7 |
| γ | |
| Escherichia colia | 8 |
| Pseudomonas fluorescens | 9 |
| Pseudomonas aureofaciens | 9 |
| Serratia plymuthicaa | 9 |
| Xanthomonas campestris | 9 |
| Enterobacter cloacae | 9 |
| Azotobacter vinelandii | 4 |
| Klebsiella pneumoniae | 10 |
| δ | |
| Desulfovibrio desulfuricansa | 11 |
| Gram positive | |
| Low G+C | |
| Bacillus cereus | 9 |
| High G+C | |
| Arthrobacter oxydans | 12 |
| Arthrobacter globiformisa | 12 |
| Streptomyces bikiniensis | 12 |
| Streptomyces coelicolor | 12 |
| Streptomyces griseus | 12 |
DNA from this organism was used for routine probe calibration.
Sources were as follows: 1, J. Parke (University of Wisconsin-Madison); 2, P. Weimer (University of Wisconsin-Madison); 3, American Type Culture Collection (ATCC; Rockville, Md.) strain 29145; 4, G. Roberts (University of Wisconsin-Madison); 5, J. Lindquist (University of Wisconsin-Madison); 6, S. Fetzner (University of Hohenheim); 7, R. C. Wyndham (Carleton University); 8, authors’ lab collection; 9, R. Zablotowicz (U.S. Department of Agriculture-ARS, Stoneville, Miss.); 10, B. Thede (Ciba-Giegy Corp., Greensboro, N.C.); 11, ATCC strain 27774; and 12, J. Ensign (University of Wisconsin-Madison).