Table 2.
Cumulative yield of nucleotides sequenced in time.
| Sample | Virus/viroid | Nucleotides | Time | Nucleotides | Time | Nucleotides | Time | Nucleotides | Time |
|---|---|---|---|---|---|---|---|---|---|
| number | present | (millions) | (minutes) | (millions) | (minutes) | (millions) | (minutes) | (millions) | (minutes) |
| MinION direct RNA | MinION direct RNA sequencing | MinION cDNA-PCR | MinION cDNA-PCR sequencing | ||||||
| sequencing of totRNA | of rRNA-depleted totRNA | sequencing of totRNA | of rRNA-depleted totRNA | ||||||
| I | TYLCV | 500 | 520 | / | / | 30 | 50 | 50 | 220 |
| ToCV | / | / | 30 | 80 | 50 | 130 | 10 | 20 | |
| PepMV | 10 | 10 | 10 | 30 | 10 | 10 | 10 | 20 | |
| ToMV | / | / | 50 | 140 | 10 | 10 | 10 | 20 | |
| STV | / | / | 30 | 80 | / | / | 100 | 600 | |
| CLVd | 700 | 710 | 30 | 80 | 30 | 50 | 10 | 20 | |
| II | CaMV | 10 | 10 | NA | NA | NA | NA | NA | NA |
| CCyV1 | / | / | NA | NA | NA | NA | NA | NA | |
| III | TSWV | 20 | 30 | NA | NA | NA | NA | NA | NA |
| IV | TASVd | 200 | 220 | NA | NA | NA | NA | NA | NA |
| V | PVeV2 | 200 | 170 | NA | NA | NA | NA | NA | NA |
Data in table represent the time when enough nucleotides would be sequenced to cover at least 50% of virus/viroid reference sequence by mapping reads. / no data: 50% coverage of virus/viroid reference by reads were not achieved. NA, not applicable.