Figure 3.

Intraperitoneal injection of synthetic PSM⍺3 fibrillized with CpG DNA elicits an autoantibody response. (A) NZBxW/F1 or (B) Balb/c mice were injected bi-weekly with PSMα3 fibrillzed alone (black bar) or fibrillzed in the presence of CpG DNA (blue bar) or control mice were injected with sterile PBS (gray bar). Mice were tail bled weekly and the production of anti-dsDNA autoantibodies were detected (Optical density 650-405nm). Black dotted line represents the maximum anti-dsDNA autoantibody production of positive control sera and the red dotted line represent autoantibody detection in naïve serum. Mean and SEM graphed; significance was calculated using a 2-way ANOVA and Tukey’s multiple comparisons tests (*, P < 0.05).