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. 2022 May 11;16:886634. doi: 10.3389/fnbeh.2022.886634

FIGURE 3.

FIGURE 3

Persistent effects of ethanol on tau and Aβ proteins in female 3xTg-AD mice. Adult female 3xTg-AD mice received either EtOH (5g/kg/d, i.g., 5d on 2d off, N = 8) or the same schedule of water (i.g., N = 4) for 3 months (P168–P264), followed by prolonged abstinence until 14 months. Pathologic tau and Aβ protein levels were measured in cortex and hippocampus by western blot. (A,B) Tau species in cortex. In the cortex EtOH caused a persistent increase in (A) total tau, 36% (N = 3 control, 8 ethanol, 1 outlier) and (B) phosphorylated tau214, p-tau214, 50%, *p < 0.05. (C,D) Tau in hippocampus. In the hippocampus, EtOH persistently increased levels of (C) total Tau, 23% increase, *p < 0.05 without altering (D) p-tau214, p = 0.9 levels. (E,F) Aβ1–42 levels. EtOH had no persistent impact on levels of Aβ1–42 protein in female cortex, p = 0.4 (E), though a (F) 30% increase was found in the hippocampus. *p < 0.05, **p < 0.01, t-test.