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. 2022 May 11;16:886634. doi: 10.3389/fnbeh.2022.886634

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Copyright © 2022 Tucker, Alicea Pauneto, Barnett and Coleman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Chronic binge ethanol has no persistent effect on major regulators of tau phosphorylation in female 3x-TgAD mice. Adult female 3xTg-AD mice received EtOH (5g/kg/d, i.g., 5d on 2d off) for 3 months (P168–P264), followed by prolonged abstinence until 14 months. Common tau-phosphorylating kinases (GSK3β and PKA) and the tau de-phosphorylating enzyme PP2A were measured by Western Blot. (A) Total GSK3β protein level in cortex was unchanged by ethanol. (B) Levels of active p-Tyr216 GSK3β were unchanged by ethanol and levels of the inactive p-Ser9-GSK3β showed a trend toward a 20% reduction, p = 0.09. (C) Ethanol caused a slight 5% increase in protein kinase A (PKA), *p < 0.05. (D) Ethanol had no effect on expression levels of PP2A. N = 4 control, 8 ethanol. *p < 0.05, t-test.