TABLE 1.
Protein yield and chloride release by recombinant strains VP44(pE43) and VP44(pPC3) grown on CBAs and CBs
| Substrate (mM) | 44(pE43)
|
44(pPC3)
|
||
|---|---|---|---|---|
| Protein (μg/ml) (±SD) | Chloride (mM) (±SD) | Protein (μg/ml) (±SD) | Chloride (mM) (±SD) | |
| Benzoate (5) | 181 (39) | 0.4 (0.1) | 228 (24) | 0.7 (0.1) |
| 2-CBA (5) | 169 (15) | 5.3 (0.4) | <10 | <0.1 |
| 2-CBA (10) | 373 (16) | 10.1 (1.2) | <10 | <0.1 |
| 4-CBA (5) | <10 | <0.1 | 158 (9) | 7.1 (0.2) |
| 4-CBA (10) | <10 | <0.1 | 257 (16) | 10.4 (0.1) |
| Biphenyl (2) | 154 (10) | 0.5 (0.1) | 188 (9) | 0.1 (0.1) |
| 2-CB (2) | 177 (4) | 1.9 (0.2) | 69 (7)b | 0.1 (0.1) |
| 4-CB (2) | 84 (6)a | 0.7 (0.3) | 160 (39) | 1.8 (0.2) |
a
4-CBA accumulated to 2.02 (±0.21) mM in the supernatant.
b
2-CBA accumulated to 1.88 (±0.13) mM in the supernatant.