Fig. 1.

Sortilin promotes apo(a) secretion. HepG2 cells stably transfected with a 17-kringle form of apo(a) were transiently transfected with an expression vector encoding sortilin, the corresponding empty vector (pcDNA), or SORT1 siRNA or a scrambled siRNA. The cells were subjected to a pulse-chase protocol followed by immunoprecipitation of medium and lysate samples using an anti-apo(a) antibody. Representative fluorographs are shown in (A). Densitometry was performed for lysate (B) and media (C) fluorographs. The data shown are the means ± SEM of at least three independent experiments. Significance compared to pcDNA is indicated through asterisks, where ∗P<0.05; ∗∗P<0.01; ∗∗∗P<0.001. Significance compared to sortilin is indicated through daggers, where †P<0.05; ††P<0.01; †††P<0.001; ††††P<0.0001. The colors correspond to the plots. apo(a): apolipoprotein(a).