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. 2022 Apr 24;12(8):3584–3600. doi: 10.7150/thno.72192

Figure 4.

Figure 4

Overexpression of myeloid Trib1 reduces hypoxic TAM numbers in the TME and inhibits TAM polarization towards a pro-inflammatory phenotype. Post-mortem analysis of TAMs and their subtypes based on the location and phenotypes using immunofluorescence staining. (A) Representative images of perivascular TAM CD31 (white) and F4/80 (green) (Scale: 50 µm), (D) hypoxic TAM CA9 (red) and F4/80 (green) (Scale: 100 µm), (F) pro-inflammatory TAM CD31 (white), NOS2 (red), and F4/80 (green) (Scale: 50 µm), and (I) anti-inflammatory TAM CD31 (white), MR (red), and F4/80 (green) (Scale: 50 µm) in Trib1mWT and Trib1mTg tumors. Cells were quantified manually from 4-5 randomly taken fields of view using ImageJ. Percentage of TAMs and TAMs classified based on their location (vessels and hypoxia) were Trib1 overexpression inhibited TAMs, both perivascular and hypoxic TAMs, pro-inflammatory TAMs and pro-inflammatory TAMs in the vessels in tumors compared to WT (B, C, E, G, H respectively). Percentage of anti-inflammatory TAMs did not alter in Trib1mTg (J, K). Results of unpaired t-test are presented; mean±SEM is plotted; *p < 0.05 **p < 0.01 (n = 5-9 mice/group).