Figure 2.

Generation and verification of Mecp2^Ksp KO mice. (A) Experimental strategy to generate Mecp2^Ksp KO mice. Blue boxes indicate exons of Mecp2, red triangles indicate LoxP site. (B) Representative genotyping results. (C) The mRNA levels of Mecp2 in WT and Mecp2^ksp KO mice with or without IR injury. (D) Representative immunohistochemical staining of Mecp2 in the kidney of WT and Mecp2^ksp KO mice with or without IR injury; Scale bar = 100 µm. Brown color indicates positive staining. (E-G) Representative co-immunofluorescent staining for Mecp2 (green) with PNA (red; E) or LTL (red; F) in the kidney of WT and Mecp2^ksp KO mice at day 3 after IR injury (IR3D), and quantitative results of the percentage of Mecp2⁺ cells in PNA⁺ (G, left) or LTL⁺ (G, right) tubular cells. DAPI (blue) stained nuclei; Scale bar = 50 µm. n = 3-6 per group. *P < 0.05.