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Confirmation of expression of stem cell markers in hESC (MAN13) cells. Representative immunofluorescence of pluripotency markers in MAN13 hESCs with primary antibodies against: NANOG, OCT4, SOX2, SSEA3, SSEA1, TRA1-81 and TRA1-60. Goat anti-rabbit Alexa Fluor plus 488 antibody was used to detect primary antibodies. DAPI was used for nuclear staining. (N = 3; scale bars = 100 µm).
