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Lack of circadian rhythm in hESC (MAN13) cells. (A). Fluorescence and phase contrast images after lentiviral transduction of MAN13 cells with hBMAL1-Luc or hPER2-Luc plasmids, scale bar = 100 µm. Cells were sorted by FACS based on GFP signal intensity (bottom panel). (B). Bioluminescence traces of hBMAL1-Luc and hPER2-Luc reporters in dexamethasone treated GFP sorted MAN13 cells (N = 3 for each reporter).
