Figure 6.

Expression of clock proteins are readily detectable in hESCs. (A). Representative IF of clock factors in hESCs. MAN13 cells were fixed and incubated with antibodies against CLOCK, BMAL1, PER2 and CRY1, followed by Alexa Fluor plus 488 antibody. Nuclei were stained with DAPI. Scale bars represent 100 μm. Representative images from N = 3 except CRY1 (N = 2). (B). Co-expression of CLOCK (in green) and OCT4 (in red) proteins in MAN13 hESCs by IF. Nuclei were stained with DAPI. Scale bar = 100 μm. Representative images of N = 2.