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. 2022 May 1;12(8):3690–3702. doi: 10.7150/thno.70841

Figure 6.

Figure 6

In vitro therapy. (A and B) Relative cell viabilities of 4T1 cells treated by 9T-PUNNC at different concentrations and pH values without (A) and with (B) NIR-II irradiation. (C) Comparison of therapeutic efficacies of the 9T-PUNNC as a synergistic PTT and CDT agent with single PTT, CDT and additive treatment. (D) Confocal fluorescence images of DCFH-DA stained 4T1 cells after treated with the 9T-PUNNC for 20 min at pH = 5.0 with NIR-II irradiation. (E) Confocal fluorescence images of calcein-AM/PI double-stained 4T1 cells after treated with the 9T-PUNNC at pH = 5.0 with NIR-II irradiation. The scale bar is 50 µm. Statistical analysis was performed using the unpaired Student's t-test with *P < 0.05, ** P < 0.01 and *** P < 0.001 considered as statistically significant.