Figure 4.

In vivo therapeutic effect of Ti₃C₂ NSs on DSS-induced colitis. (A) The scheme shows the protocol of DSS-induced colitis and the oral administration of Ti₃C₂ NSs for IBD treatment. (B) Daily body weight changes of mice from different groups as indicated. (C-D) Mice were sacrificed on day 10, and colons were collected, imaged, and their lengths measured. (E) Microscopy images of H&E-stained of longitudinal sections of these colon after different treatments. (F) Confocal images of colon tissue stained by DCFH-DA from various groups. (G) Bio-TEM of colon tissue from these mice after Ti₃C₂ NSs oral administration (Green, DCF; WGA, Wheat Germ Agglutini, Red; DAPI, blue). (H) The Ti levels in the colon tissue determined by ICP-OES at different days. (I) The Ti contents in the neutral and positively charged film after incubation with Ti₃C₂ solutions (The insert is the corresponding photograph). The statistical significance was calculated by two-tailed student's t test. *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001.