TABLE 2.
Purification of enterocin B produced by E. faecium BFE 900 in 3:1 APT broth culture at 30°C
| Method | Fraction | Volume (ml) | Total activity (AU) | Activity recovery (%) | Total protein (mg) | Sp act (AU/mg) | Purification (fold) |
|---|---|---|---|---|---|---|---|
| Culturing and harvesting of supernatant | I | 3,000 | 1.92 × 107 | 100 | 1.32 × 105 | 1.46 × 102 | 1 |
| Hydrophobic interaction chromatography, rotary evaporation | II | 100 | 1.02 × 107 | 53 | 3.61 × 102 | 2.82 × 104 | 193 |
| Cation exchange, Sep Pak desalting, and freeze-drying | III | 1.5 | 3.07 × 105 | 1.6 | 2.33 | 1.32 × 105 | 904 |
| Reverse-phase HPLC of purified enterocin B | IV | 1 | 2.04 × 105 | 1 | 1.16 | 1.76 × 105 | 1,205 |