Table 1.
Sequences of the primers and LFD probe used in this work.
| Primers | Sequences (5’-3’) | Size/bp |
|---|---|---|
| VP2-F | GTCTGCAACAGGAAATGAATC | 1658 |
| VP2-R | ATTCTGGAAACATTCTTATGC | |
| qPCR-F | GGGGAGGGCTTGGTTAGAAT | 319 |
| qPCR-R | TTGGTGGTGAGGTTGCTGAT | |
| ERA-1-F | ACACTGGACAATCAGAACAAATAACAGACTC | 227 |
| ERA-1-R | CTGTGGTAGGTTCAGTTAGTAGTTTTGGAGG | |
| ERA-2-F | ACCAACATACACTGGACAATCAGAACAAATA | 225 |
| ERA-2-R | GGTAGGTTCAGTTAGTAGTTTTGGAGGCAGT | |
| ERA-3-F | ATGCAGTACCAATTCATCTTCTAAGAACAGGA | 147 |
| ERA-3-R | TGGTAGGTTCAGTTAGTAGTTTTGGAGGCAGT | |
| crRNA-1-F | GAAATTAATACGACTCACTATAGGGTAATTTCTACTAAGTGTAGATTAAAAATAGCACCAAACCTA | 66 |
| crRNA-1-R | TAGGTTTGGTGCTATTTTTAATCTACACTTAGTAGAAATTACCCTATAGTGAGTCGTATTAATTTC | |
| crRNA-2-F | GAAATTAATACGACTCACTATAGGGTAATTTCTACTAAGTGTAGATACACAAAACCACTAAAATTA | 66 |
| crRNA-2-R | TAATTTTAGTGGTTTTGTGTATCTACACTTAGTAGAAATTACCCTATAGTGAGTCGTATTAATTTC | |
| crRNA-3-F | GAAATTAATACGACTCACTATAGGGTAATTTCTACTAAGTGTAGATTAAAAACAATCCACCAGGAC | 66 |
| crRNA-3-R | GTCCTGGTGGATTGTTTTTAATCTACACTTAGTAGAAATTACCCTATAGTGAGTCGTATTAATTTC | |
| Probe | 6-FAM-TTTTTT-BHQ1 | |
| 6-FAM-TTTTTTTATTTTTTT-Biotin |