Figure 2.

Biocatalytic reductive amination for the preparation of secondary and tertiary allylic amines and their saturated analogues. Reaction conditions as specified in the scheme. Amines a, b, c, d, and g were supplied as 2 equiv, whereas amines e and h were supplied as 4 equiv. Reactions were run in 50 mM NaPi buffer, pH 7.5, incubated for 6 h at 30 °C, 250 rpm shaking. Only 0.5–8% alcohol product was detected. Enzymes: pIR23 = C. ferrugineus reductive aminase; GDH = Bacillus subtilis glucose dehydrogenase.