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. 2022 May 25;20:189. doi: 10.1186/s12916-022-02391-4

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Role of EZH2-mediated H3K27me3 in the regulation of ADAM12-S expression during syncytialization of human trophoblasts. A HE staining showed the fusion of mononuclear cytotrophoblasts into multinuclear syncytiotrophoblasts with incubation time. Scale bars, 20 μm. B Increased CGB3, GCM1, ERVW-1 and decreased CDH1 mRNA abundance, the syncytialization markers, in cultured trophoblasts upon syncytialization (n = 4). C Decreased EZH2 mRNA (n = 4) and protein (n = 6) abundance during syncytialization. D, E Increased ADAM12S mRNA (n = 4) and protein (n = 5) abundance in trophoblasts and secreted ADAM12-S abundance in the culture medium (n = 5) of trophoblast during syncytialization. F–H siRNA-mediated knockdown of EZH2 expression increased the abundance of ADAM12S mRNA (n = 3) and protein (n = 4) in trophoblasts as well as secreted ADAM12-S protein in the culture medium of trophoblasts (n = 3). Randomly scrambled siRNA served as negative control (NC). Top panels of C–H are representative immunoblots. I Decreased enrichment of EZH2 and H3K27me3 at ADAM12 promoter upon syncytialization of cultured trophoblasts (n = 3). Top panel of I illustrates aligning positions of primers used in the ChIP assay. TSS, transcription start site. Data are means ± SEM. *P < 0.05, **P < 0.01 vs 3 hrs or NC