Fig. 2.

^99mTc-HYNIC-mAb_Nectin-4 has high Nectin-4-targeting in vitro and in vivo. a Cellular uptake assays of ^99mTc-HYNIC-mAb_Nectin-4 at 1−8 h (n = 4), ***p < 0.001. b ^99mTc-HYNIC-mAb_Nectin-4 SPECT/CT imaging of xenograft tumor-bearing mice at different time points, each subgraph includes MIP (upper) and transaxial (lower) image. c The H&E and Nectin-4 immunohistochemistry staining results of xenograft tumor tissues, scale bar = 100 μm. d Biodistribution of ^99mTc-HYNIC-mAb_Nectin-4 in different organs and tumors of tumor-bearing mice at 36 h p.i.. ***p < 0.001 (n = 3). e The corresponding T/M and T/B ratio from biodistribution, *p < 0.05, ** < 0.01, ***p < 0.001