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. 2022 May 25;20:243. doi: 10.1186/s12951-022-01444-3

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© The Author(s) 2022, corrected publication 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — The optical characterization and photothermal property of mAb_Nectin-4-ICG. a The UV − vis absorbance spectra. b ICG standard concentration-absorbance curve at 790 nm. c Stability test of mAb_Nctin-4-ICG at 0–48 h. d Photothermic heating curves of mAb_Nectin-4-ICG solutions containing different ICG concentrations with laser irradiation (1.0 W/cm²). e Photothermic heating curves of a mAb_Nectin-4-ICG (20 μg/mL ICG concentration) solution irradiated with 808 nm laser following graded power densities. f Photothermal stability of mAb_Nectin-4-ICG solution over 4 repeated laser on (20 μg/mL ICG concentration, 1.0 W/cm², 3 min) and laser off cycles. g The corresponding thermal images for d