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. 2022 May 25;20:243. doi: 10.1186/s12951-022-01444-3

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© The Author(s) 2022, corrected publication 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — The mAb_Nectin-4-ICG has excellent tumor-specific targeting and retention in Nectin-4-overexpression tumor in vivo. a FL imaging for MDA-MB-468 xenografts tumor-bearing mice administrated with mAb_Nectin-4-ICG, free ICG or saline. Red circles indicates the tumor sites and green circles indicates the normal muscles (n = 3). b Ex vivo FL imaging for isolated organs/tumors at 48 h p.i. c Semi-quantitative assays of FL signal intensity for b, ****p < 0.0001 (n = 3)