Fig. 4.

XGB-induced DCA increased activates Wnt-β-catenin signal pathway and suppressed the expression of FXR. a–c IHC staining of paraffin-embedded sections of normal and colon tissues was shown. The right pictures are the quantitative analysis of FXR, β-catenin, c-Myc, and cyclinD1 proteins in tissues (Normal, HGIEN, and Colon cancer) and groups (sham and XGB). d qRT-PCR was used to detect the mRNA level of FXR, β-catenin, c-Myc, cyclinD1, and Axin2 in sham and XGB groups and different CC cells, and the expression level of FXR, β-catenin, c-Myc, and cyclinD1 were higher in XGB group and DCA-treated CC cells than sham group and control cells. e, f Western blotting was used to detect the protein level of β-catenin, FXR, c-Myc, VGEF, cyclinD1, and CDK1 in sham and XGB groups and different CC cells. g Western blotting was used to detect the protein level of cytosolic β-catenin in different CC cells. *P < 0:05, **P < 0.01, ***P < 0.001