Fig. 5.

OARSI scores, based on MIA dose. a–b With 0.25 mg and 0.5 mg of MIA, OARSI scores increased in a time-dependent manner, except for the score at 1–2 weeks in the 0.25-mg MIA group. c–d With 1.0 mg and 2.0 mg of MIA administration, time-dependent increases in OARSI scores were observed until 4 weeks after MIA injection. e With 4.0 mg of MIA administration, OARSI scores reached the maximum after 4 weeks. There were no significant differences among the four-week, eight-week, and 12-week groups. Data are presented as mean ± SEM, n = 6 rats per group. Data were analyzed using two-way ANOVA, followed by the Tukey–Kramer method. **, p < 0.01 (vs. 1 week group). §§, p < 0.01 (vs. two-week group). ¶¶, p < 0.01 (vs. four-week group). ††, p < 0.01 (vs. eight-week group). Abbreviations: OARSI, Osteoarthritis Research Society International; MIA, monoiodoacetate; SEM, standard error of the mean