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Replication of different ZIKV strains at suboptimal temperatures. Replication rates for SPH (A), MR-766 (B), and IbH (C) at cool temperature. The cells were infected at an MOI of 0.1 for 2 h at 28°C and incubated at either 20°C or 28°C for 10 days. Supernatants were collected every 48 h and titrated. Dashed lines represent the limit of detection. Data shown are means ± SEM of results from five replicates for each virus.
