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. 2022 May 2;96(10):e00070-22. doi: 10.1128/jvi.00070-22

TABLE 1.

Primer and siRNA sequences used in this study

Purpose and name Sequence (5′–3′)
Real-time PCR Primers
 PEDV N forward GAGGGTGTTTTCTGGGTTG
 PEDV N reverse CGTGAAGTAGGAGGTGTGTTAG
 pTARDBP forward TTTTGCCTTTGTTACATTTGC
 pTARDBP reverse AGTTCATCCCTCCACCCAT
 KLF16 forward CCTTTGCTTGCGACTGG
 KLF16 reverse CGAGCGTGCTTGGTCAG
 ACTB forward TCCCTGGAGAAGAGCTACGA
 ACTB reverse AGCACTGTGTTGGCGTACAG
 pGAPDH forward ATGGATGACGATATTGCTGCGCTC
 pGAPDH reverse TTCTCACGGTTGGCTTTGG
 hIFN-β forward TCTTTCCATGAGCTACAACTTGCT
 hIFN-β forward GCAGTATTCAAGCCTCCCATTC
ChIP assay
 ChIP primer forward CCAGTCTCGGGAGGGTCCAGAGGGC
 ChIP primer reverse GCCCTGGGTCTCGGCCGGAGCTCGC
siRNA sequences
 si-TARDBP sense GGCCUUUGGUUCUGGAAAUTT
 si-TARDBP antisense AUUUCCAGAACCAAAGGCCTT
 si-KLF16 sense CUGCCAAAGAGCAGUUUAATT
 si-KLF16 antisense UUAAACUGCUCUUUGGCAGTT
 si-MyD88 sense GUACAAGGCAAUGAAGAAATT
 si-MyD88 antisense UUUCUUCAUUGCCUUGUACTT
 si-TRAF3 sense GGCCGUUUAAGCAGAAAGUTT
 si-TARF3 antisense ACUUUCUGCUUAAACGGCCTT
 si-TRAF6 sense GCGCUGUGCAAACUAUAUATT
 si-TRAF6 antisense UAUAUAGUUUGCACAGCGCTT
 NC sense UUCUCCGAACGUGUCACGUTT
 NC antisense ACGUGACACGUUCGGAGAATT