TABLE 1.
Purpose and name | Sequence (5′–3′) |
---|---|
Real-time PCR Primers | |
PEDV N forward | GAGGGTGTTTTCTGGGTTG |
PEDV N reverse | CGTGAAGTAGGAGGTGTGTTAG |
pTARDBP forward | TTTTGCCTTTGTTACATTTGC |
pTARDBP reverse | AGTTCATCCCTCCACCCAT |
KLF16 forward | CCTTTGCTTGCGACTGG |
KLF16 reverse | CGAGCGTGCTTGGTCAG |
ACTB forward | TCCCTGGAGAAGAGCTACGA |
ACTB reverse | AGCACTGTGTTGGCGTACAG |
pGAPDH forward | ATGGATGACGATATTGCTGCGCTC |
pGAPDH reverse | TTCTCACGGTTGGCTTTGG |
hIFN-β forward | TCTTTCCATGAGCTACAACTTGCT |
hIFN-β forward | GCAGTATTCAAGCCTCCCATTC |
ChIP assay | |
ChIP primer forward | CCAGTCTCGGGAGGGTCCAGAGGGC |
ChIP primer reverse | GCCCTGGGTCTCGGCCGGAGCTCGC |
siRNA sequences | |
si-TARDBP sense | GGCCUUUGGUUCUGGAAAUTT |
si-TARDBP antisense | AUUUCCAGAACCAAAGGCCTT |
si-KLF16 sense | CUGCCAAAGAGCAGUUUAATT |
si-KLF16 antisense | UUAAACUGCUCUUUGGCAGTT |
si-MyD88 sense | GUACAAGGCAAUGAAGAAATT |
si-MyD88 antisense | UUUCUUCAUUGCCUUGUACTT |
si-TRAF3 sense | GGCCGUUUAAGCAGAAAGUTT |
si-TARF3 antisense | ACUUUCUGCUUAAACGGCCTT |
si-TRAF6 sense | GCGCUGUGCAAACUAUAUATT |
si-TRAF6 antisense | UAUAUAGUUUGCACAGCGCTT |
NC sense | UUCUCCGAACGUGUCACGUTT |
NC antisense | ACGUGACACGUUCGGAGAATT |