Fig. 1. The chemical reporter strategy for studying protein lactylation. (A) Chemical structure of histone lysine lactylation. (B) Schematic for the detection and identification of lactylated proteins using the bioorthogonal chemical reporter. The alkynyl-functionalized l-lactate analogue (i.e., YnLac) is metabolically incorporated into lactylated proteins in live cells. Then alkynyl-labelled proteins are conjugated with fluorescent or affinity tags for in-gel fluorescence detection or proteomic identification, respectively.
