Table 2.
Number of Mannheimia haemolytica (M. haemolytica) isolates recovered from nasopharyngeal swabs (NPS) from 11 cattle.
| Animal ID | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Plate number | 205 | 232 | 260 | 277 | 256 | 262 | 56–8 | 53–49 | 49–16 | 54–8 | 54–211 |
| 1 | 8 | 0 | 4 | 14 | 13 | 6 | 8 | 17 | 0 | 10 | 9 |
| 2 | 11 | 0 | 7 | 15 | 5 | 0 | 19 | 19 | 4 | 11 | 13 |
| 3 | 7 | 0 | 10 | 15 | 11 | 0 | 12 | 18 | 3 | 8 | 12 |
| 4 | 16 | 0 | 9 | 14 | 15 | 0 | 6 | 20 | 2 | 2 | 13 |
| 5 | 6 | 1 | 11 | 12 | 15 | 1 | 11 | 20 | 1 | 5 | 13 |
| Total M. haemolytica Isolates | 48 | 1 | 41 | 70 | 59 | 7 | 56 | 94 | 10 | 36 | 60 |
| Number of AMR phenotypes | 1 | 1 | 3 | 2 | 1 | 1 | 2 | 2 | 1 | 1 | 1 |
| M. haemolytica genotype | 1 | 1 | 2 | 2 | 1 | 2 | 2 | 2 | 2 | 2 | 2 |
Two NPS (one from each nostril) were together streaked onto the first quadrant of 5 consecutive blood agar plates (plate numbers 1 through 5), then the remaining three quadrants of each plate were streaked with a new sterile loop. Each individual colony (up to 20 for each plate) was subcultured once for determination of minimum inhibitory concentrations for 11 antimicrobials to determine AMR phenotype, and for MALDI-TOF MS to determine genotype.