FIG. 1.

Degradation of CHAM by B. oxydans IH-35. Increases in cell density (○) were determined photometrically at 660 nm. The disappearance of CHAM (●) was measured by GLC. The GLC analysis was carried out with a gas chromatograph (model GC-14A; Shimazu) equipped with a 30-m type DB-1 glass capillary column. The column was operated at 60°C, and the temperature was programmed to increase to 220°C at a rate of 10°C/min. The flow rate of the carrier gas, He, was 1.36 ml/min. The CHAM peak (retention time, 2.55 min) was detected with a flame ionization detector. Accumulation of CHnone (▵) was determined by HPLC as described in the text. OD₆₆₀, optical density at 660 nm.