TABLE 2.
Reaction stoichiometry for production of CHnone and formation of ammonia during deamination of CHAM by cell extracts of B. oxydans IH-35Aa
| Amt of CHAM added (μmol) | Production of CHnone
|
Formation of ammonia
|
||
|---|---|---|---|---|
| Amt of CHnone produced (μmol) | CHAM added/ CHnone pro-duced ratio | Amt of NH3 formed (μmol) | CHAM added/ NH3 formed ratio | |
| 10.0 | 9.87 | 1.02 | ||
| 30.0 | 30.02 | 1.00 | ||
| 50.0 | 50.60 | 0.99 | ||
| 50.0b | 0 | |||
| 2.50 | 2.58 | 0.97 | ||
| 5.00 | 4.98 | 1.01 | ||
| 10.00 | 9.58 | 0.96 | ||
| 10.00b | 0 | |||
a
Each reaction mixture contained, in a volume of 1.0 ml, 450 μmol of sodium-potassium phosphate buffer (pH 7.2) and 1.26 mg of 48,000-×-g supernatant. Reactions at 30°C were started by adding limited amounts of CHAM. The CHnone concentrations were determined by HPLC. The NH3 concentrations were determined by the method of Fawcett and Scott (5). The data are averages of the data from three different experiments.
b
Boiled-extract control.