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. 2022 Feb 7;32(6):518–528. doi: 10.1093/glycob/cwac004

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Fig. 3 — Enzyme assays of cell lysates from control HEK 293 cells and HEK 293 cells overexpressing NDST1A or NDST1B. The assays were carried out on similar volumes of lysates of stable cell lines expressing NDST1A (A1, A2) and NDST1B (B1 and B2). The values given have been corrected for protein content of the lysates. (A) N-deacetylase ELISA assay of serial dilutions of cell lysates prepared from HEK 293 cells transfected with empty vector (•), or from cells stably overexpressing NDST1A () and NDST1B (), respectively. (B) N-sulfotransferase activity and (C) combined N-deacetylase and N-sulfotransferase activities. The values shown are the mean of duplicates.

Inline graphic — Enzyme assays of cell lysates from control HEK 293 cells and HEK 293 cells overexpressing NDST1A or NDST1B. The assays were carried out on similar volumes of lysates of stable cell lines expressing NDST1A (A1, A2) and NDST1B (B1 and B2). The values given have been corrected for protein content of the lysates. (A) N-deacetylase ELISA assay of serial dilutions of cell lysates prepared from HEK 293 cells transfected with empty vector (•), or from cells stably overexpressing NDST1A () and NDST1B (), respectively. (B) N-sulfotransferase activity and (C) combined N-deacetylase and N-sulfotransferase activities. The values shown are the mean of duplicates.