Skip to main content
. 2022 Feb 7;32(6):518–528. doi: 10.1093/glycob/cwac004

Fig. 5.

Fig. 5

HS and CS disaccharide composition. Disaccharide composition of HS (A and B) and CS (C) produced by HEK 293 cell lines overexpressing NDST1A (light gray bars) or NDST1B (dark gray bars) or transfected with empty vector (black bars) analyzed by RPIP-HPLC. The values for the different cell lines shown are the mean of triplets ± SD. An asterisk * indicates a significant difference (P < 0.05), calculated using the 2-tailed Student’s t-test. (A) HS disaccharide composition: D0A0, HexAGlcNAc; D0S0, HexAGlcNS; D0A6, HexAGlcNAc(6S); D2A0, HexA(2S) GlcNAc; D0S6, HexAGlcNS(6S); D2S0, HexA(2S)GlcNS; D26S, HexA(2S) GlcNS(6S). (B) Percentage of unsulfated disaccharides (OS), N-sulfated disaccharides (NS), 6-O-sulfated disaccharides (6S), and 2-O-sulfated disaccharides (2S). Total sulfation is the sum of N-sulfate, 2-O-sulfate, and 6-O-sulfate groups in 100 disaccharides. (C) CS disaccharide composition: D0a0: HexAGalNAc; D0a4: HexAGalNAc(4S); D0a6: HexAGalNAc(6S); D2a4: HexA(2S)GalNAc(4S); D0a10 HexAGalNAc4S6S. Disaccharide abbreviations are according to the published structural code in Lawrence et al. (2008).