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. Author manuscript; available in PMC: 2022 May 25.
Published in final edited form as: Biotechnol Bioeng. 2020 Jun 17;117(9):2816–2826. doi: 10.1002/bit.27441

FIGURE 6.

FIGURE 6.

Contribution of edited mESCs to mouse embryonic development. (a) A chimeric mouse containing edited mESCs. Chimeric mice were generated by injection of heterozygous TC1 (hm/m) into mouse blastocysts (C57/BL6 albino). White fur color was from host embryo and gray fur was derived from injected mESCs. (b) Chimeric ratios of mESCs in tissues. Chimeric ratio was determined by ratio of hmTert and mTert copy number, as determined by genomic PCR analyses using primers specific for hmTert and mTert alleles, respectively. The ratio of mESCs (hm/m) was defined as 100%.