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Efficiencies of Crispr/Cas9 assisted homologous recombination at the mTert locus in mESCs
| Cell lines | # of cells | # of PuromycinR colonies | # of colonies isolated | GCVR clones | Recombination at the 5’ end | TKneo+ by PCR | % of colonies via HR |
|---|---|---|---|---|---|---|---|
|
| |||||||
| TC1 | 3.5 × 106 | 3400 | 800 | 110 | 84/800 (11%) | 16/110 (15%) | 84/110 (76%) |
| v6.5 | 2.2 × 106 | 1500 | 800 | 156 | 130/800 (16%) | 22/156 (14%) | 130/156 (83%) |
