. Author manuscript; available in PMC: 2022 May 25.

Published in final edited form as: Biotechnol Bioeng. 2020 Jun 17;117(9):2816–2826. doi: 10.1002/bit.27441

TABLE 4.

Aberrations at the cleavage sites of the mTert allele in hm/m mESC clones

mESC clones	Sequences at the 5’ cleavage site (5’−3’)	Sequences at the 3’ cleavage site (5’−3’)

v6.5	GGAAAGGATGAGGTTGGGCCAATGGGCTA	GACTCTGCAATGGCGTGGTCCCACGGTGTTCTG
hm/m #1	GGAAAGGATGAGGTT-----------------GGGCTA	GACTCTGCAATGGCGTGGTCCCACGGTGTTCTG
hm/m #2	GGAAAGGATGAGGTTGGGC--AATGGGCTA	GACTCTGCAATGGCGTGG-----------------------TCTG
hm/m #3	GGAAAGGATGAGGTTGGGCC--ATGGGCTA	GACTCTGCAATGGCGTGGTCCCACGGTGTTCTG
hm/m #4	GGAAAGGATGAGGTTGGGC--AATGGGCTA	GACTCTGCAATGGCGTGGT----CACGGTGTTCTG
hm/m #5	GGAAAGGATGAGGTTGGGCCAATGGGCTA	GACTCTGCAATGGCGTGGTCCCACGGTGTTCTG
hm/m #6	GGAAAGGATGAGGTTGGGCC--ATGGGCTA	GACTCTGCAATGGCGTGG----CCACGGTGTTCTG

Shaded sequences of V6.5 indicated the sgRNA targeted sequences.