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. 2022 May 24;60(1):846–853. doi: 10.1080/13880209.2022.2066700

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — The effects of EMS on the expression of M1 and M2 marker proteins iNOS and Arg-1 in PMs of AA rats. (A) Western blotting was used to detect the expressions of iNOS and Arg-1 in PMs of AA rats. (B, C) Densitometric analyses of iNOS and Arg-1 normalized to histone β-actin. The effects of EMS on the CD86/CD206 ratio and phagocytic ability. (D) CD86, CD206 and phagocytic ability were detected by flow cytometry. (E) The ratio of CD86/CD206 in PMs of AA rats. (F) The mean fluorescence intensity of phagocytic ability was calculated. (G) The levels of TNF-α, IL-1β, TGF-β and IL-10 were detected by ELISA. ##p < 0.01 vs. the normal group; *p < 0.05; **p < 0.01 vs. the model group (n = 3).