Skip to main content
. 2022 May 19;60(1):990–996. doi: 10.1080/13880209.2022.2067572

Figure 2.

Figure 2.

Calycosin elicits its effects through the inhibition of ferroptosis. (A–C) HK-2 cells were treated with erastin (35 μM) and/or calycosin (40 μM). Then, the cell viability (A), lipid ROS levels (B) and NOCA4 protein levels (C) were detected. ***p < 0.001 vs. vehicle; ##p < 0.01, ###p < 0.001 vs. erastin. (D) Three knockdown lentiviruses against GPX4 (shGPX4-1, shGPX4-2 and shGPX4-3) were transfected to HK-2 cells, and GPX4 expression level was measured by real-time PCR and Western blot. ***p < 0.001 vs. shNC. (E–G) HK-2 cells were transfected with shGPX4 lentivirus and/or calycosin (40 μM). Then, the cell viability (E), lipid ROS levels (F), and NOCA4 protein levels (G) were detected. **p < 0.01, ***p < 0.001 vs. shNC; #p < 0.05, ###p < 0.001 vs. shGPX4.