|
Galleria mellonella larvae |
Fauna Topics GmbH, order number: 527 |
| Trypsin, porcine, MS grade |
Merck, order number: 650279 |
| Anti-SecG antiserum |
gift of R. Voulhoux, CNRS AMU LCB, Marseille |
| Anti-lipid A antibodies |
Acris Antibodies, Herford, Germany, order number: BP 2235 |
| Ni-NTA agarose |
Macherey–Nagel, Düren, Germany, order number: 745400 |
|
n-Octyl-β-D-glucoside |
Merck, order number: 850511P |
|
para-Nitrophenyl butyrate |
Sigma-Aldrich, order number: N9876 |
| Glycerophospholipids |
Avanti Polar Lipids, Alabaster, USA |
| NEFA-HR(2) kit |
Wako Chemicals, Richmond, USA, order number: 999-34691 |
| [N-((6-(2,4-DNP)amino)hexanoyl)-1-(BODIPYFL C5)-2-hexyl-sn-glycero-3-phosphoethanolamine] |
Thermo Fisher Scientific Inc, Waltham, MA, order number: A10070 |
| 1-O-(6-BODIPY558/568-aminohexyl)-2-BODIPYFL C5-Sn-glycero-3-phosphocholine |
Thermo Fisher Scientific Inc, Waltham, MA, order number: A10072 |
|
N-((6-(2,4-dinitrophenyl)amino)hexanoyl)-2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl)–1-hexadecanoyl-sn-glycero-3-phosphoethanolamine triethylammonium salt |
Thermo Fisher Scientific Inc, Waltham, MA, order number: D23739
|
|
Thermomyces lanuginosus PLA1
|
Sigma-Aldrich, order number: L3295 |
|
Naja mocambique mocambique PLA2
|
Sigma-Aldrich, order number: P7778 |
| Dimethyl pimelimidate |
Merck, order number: 80490 |
| Bis(sulfosuccinimidyl) glutarate |
Thermo Fisher Scientific, order number: 21610 |
| Bis(sulfosuccinimidyl) suberate |
Thermo Fisher Scientific, order number: 21586 |
| PD-10 columns |
Merck, order number: GE17-0851-01 |
| NHS Labeling Kit |
NanoTemper, Munich, Germany, order number: MO-L011 |
| 4-Methylumbelliferyl palmitate |
Sigma-Aldrich; order number: M7259 |
| CytoTox 96 non-radioactive cytotoxicity assay |
Promega, order number: G1780 |
| NucleoSpin RNA Preparation Kit |
Macherey–Nagel, Düren, Germany, order number: 740955 |
| RNase-Free DNase Kit |
Qiagen, Hilden, Germany, order number: 79254 |
| Ambion DNA-Free DNase Kit |
Thermo Fisher Scientific, Darmstadt, Germany, order number: AM1906 |
| Maxima First Strand cDNA Synthesis Kit |
Thermo Fisher Scientific, Darmstadt, Germany, Order number: K1641 |
| SYBR Green/ROX qPCR Master Mix |
Thermo Fisher Scientific, Darmstadt, Germany, order number: K0221 |
| N-Methyl-N-(trimethylsilyl) trifluoroacetamide |
Sigma-Aldrich; order number: 69479 |